The effective synergy of multiple subjects is an important way to promote the high-quality development of youth sports. By using the method of literature, comparative analysis and logic analysis, this paper explores multi-subject cooperation in promoting high-quality development of youth sports. It find that, a youth sports multiple participants development is a multi-participation pattern, a stable cooperation relationship, a good cooperation order and a systematic development synergy. Scientific positioning and labor division, effective resource allocation, and reasonable benefit distribution are keys to realizing effective collaboration among multiple subjects. Predicament of multi-subject coordinated development include institutional mechanisms for collaboration are not sound, relationships in collaboration are loose and conditions for collaboration need to be optimized. The influencing factors include: differentiated goals and demands make it difficult to establish a collaborative mechanism, the path dependence of the development subjects leads to poor sustainability, dual lack of organizational self-improvement and external incentives makes it hard to form a collaborative pattern, and insufficient policies led a low sustainability of coordinated development of multiple subjects. We should optimize top-level design of effective cooperation, clarify youth sports functions of each subject, build a collaboration and interaction platform, promote development capabilities of each subjects, and improve legal system for youth sports to optimize the collaborative level of multiple subjects in the development of youth sports